Use of short interfering RNAs (siRNAs) to investigate which equine herpesvirus type 1 (EHV-1) glycoproteins are essential for infection
Presentation
Overview
Overview
Description
The ultimate goal of our project was to identify which equine herpesvirus type 1 (EHV-1) glycoproteins are essential for productive infection. There are thirteen EHV-1 glycoproteins embedded within the lipid envelope of the virus. From a viral DNA template, several glycoprotein genes were amplified via polymerase chain reaction. Two of these genes, gK and gG, were inserted into the expression vector, LITMUS 28i and the resulting plasmid was transformed into competent E.coli. The plasmids were isolated from bacteria, purified, and then digested with specific restriction enzymes to verify that they contained gK or gG. siRNA specific for gK was generated from the plasmid and will be tested for its ability to knockdown expression of the glycoprotein K and for its ability to inhibit EHV-1 infection. Similar experiments will be performed with the other glycoproteins.
