Equus caballus MHC class I is an entry receptor for equine herpesvirus type 1 (EHV-1)
Presentation
Overview
Overview
Description
In this study, Equus caballus MHC class I was identified as a cellular entry receptor for the alphaherpesvirus, equine herpesvirus type 1 (EHV-1). This novel EHV-1 receptor was discovered using a cDNA library from equine macrophages. cDNAs from this EHV-1 susceptible cell type were inserted into EHV-1 resistant B78H1 murine melanoma cells, these cells were infected with an EHV-1 LacZ reporter virus, and cells that supported virus infection were identified by X-gal staining. Positive cells were subjected to several rounds of purification to obtain homogeneous cell populations that were shown to be uniformly infected with EHV-1. cDNAs from these cell populations were amplified by PCR and then sequenced. The sequence data revealed that the EHV-1 susceptible cells had acquired an Equus caballus MHC class I cDNA. Cell surface expression of this receptor was verified by confocal immunofluorescence microscopy and the receptor was also shown to be expressed on the EHV-1-susceptible equine dermal (ED) and chinese hamster ovary (CHO-K1) cells. The MHC class I cDNA was cloned into a mammalian expression vector and stable B78H1 cell lines were generated that express this receptor. These cell lines were susceptible to EHV-1 infection while the parental B78H1 cells remained resistant to infection.
